Determination of urea in serum by using naturally immobilized urease in a flow injection conductimetric system
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Determination of urea in serum by using naturally immobilized urease in a flow injection conductimetric system.
A flow injection method was developed, aimed at the determination of urea in human serum. The system makes use of the naturally immobilized urease present in Canavalia ensiformis DC (jack bean). A column is filled with small pieces of this bean, and the sample (50 microliters) containing urea passes through it carried by a 1% NaCl solution. On leaving the column the stream is merged with an alk...
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We used a differential thermal detector in conjunction with an immobilized urease reactor to determine urea in serum. Samples (120 mul) are introduced into a flow stream and passed through an "adiabatic" column, which is packed with enough insolubilized urease to completely convert urea to ammonia and carbon dioxide. Measured temperature changes are directly proportional to the serum urea conce...
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متن کاملDetermination of urea in serum based on the combination of an enzymatic reaction with immobilized urease and ion chromatographic analysis.
A quantitative method for the determination of urea in serum was studied. An ion chromatograph (IC) with a conductivity detector was used in this method, where the chromatograph was modified by placing an immobilized urease column between the injection loop and a guard column of the cation analysis column. Immobilized urease was prepared by the adsorption of urease on cedar sawdust with triethy...
متن کاملThe Determination of Urea in Urine by the Urease Method
Marshall’s1 urease method, in the modification proposed by Van Slyke and Cullen, is apparently the most accurate method available for the determination of urea. The method has, however, not yet attained the maximal degree of accuracy to be expected from volumetric processes in general. From the published figures of Van Slyke and Cullen, their modification appears to yield duplicates agreeing wi...
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ژورنال
عنوان ژورنال: The Analyst
سال: 1991
ISSN: 0003-2654,1364-5528
DOI: 10.1039/an9911600357